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194 vimentin  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc 194 vimentin
    194 Vimentin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 4569 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vimentin/Vimentin+XP+Rabbit+mAb/pm42880408-83-41-43
    Average 98 stars, based on 4569 article reviews
    194 vimentin - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Incubation:

    Article Title: Exosomal circ0000549 promotes MNNG-induced gastric cancer through miR-15b-5p/KIF1B
    Article Snippet: .. The membranes were blocked with 5% skimmed milk for 1 h at room temperature and incubated overnight at 4°C with primary antibodies against N-cadherin (Cell Signaling Technology, Inc; cat. no. 13116S; dilution: 1:1,000), E-cadherin (Cell Signaling Technology, Inc; cat. no. 3195S; dilution: 1:1,000), PCNA (Cell Signaling Technology, Inc.; cat. no. 13110S; dilution: 1:1,000), Nanog (Cell Signaling Technology, Inc.; cat. no. 4903S; dilution: 1:1,000), OCT4 (Cell Signaling Technology, Inc; cat. no. 2750; dilution: 1:1,000), Lin28 (Cell Signaling Technology, Inc.; cat. no. 8706S; dilution: 1:1,000), SOX2 (Cell Signaling Technology, Inc; cat. no. 23064S; dilution: 1:1,000), Vimentin (Cell Signaling Technology, Inc.; cat. no. 5741S; dilution: 1:1,000), HSP70 (Cell Signaling Technology, Inc.; cat. no. 4872S; dilution: 1:1,000), Alix (Cell Signaling Technology, Inc.; cat. no. 92880S; dilution: 1:1,000), TSG101 (Proteintech, Inc; cat. no. 28283-1-AP; dilution: 1:1,000), Calnexin (Cell Signaling Technology, Inc.; cat. no. 2679S; dilution: 1:1,000), KIF1B (Abcam; cat. no. ab72108; dilution:1:1,500) and GAPDH (Bioworld Technology, Inc.; cat. no. BS65483M; dilution: 1:2,000). .. After washing with TBST containing 0.1% Tween-20, membranes were incubated with appropriate horseradish peroxidase-conjugated secondary antibodies (Signalway Antibody LLC; cat. no. L3012; dilution: 1:5,000).

    Article Title: Multi-Omics and Functional Analyses Identify let-7b-3p as a Negative Regulator of EMT in Lung Adenocarcinoma.
    Article Snippet: Lung adenocarcinoma (LUAD) is the most common subtype of non‐small cell lung cancer (NSCLC), and its malignant progression involves complex molecular mechanisms.. While microRNAs (miRNAs) play a crucial regulatory role in LUAD development, their specific mechanisms remain unclear.. This study used bioinformatics analysis to identify key miRNA‐mRNA interaction axes in LUAD, revealing that let‐7b‐3p was significantly downregulated.

    Article Title: Exosomal circ55033 promotes cigarette smoke-induced bladder cancer by modulating EMT and stem cell properties.
    Article Snippet: 1 Department of Urology, The Second Affiliated Hospital of Anhui Medical University, Hefei 230032, China 2 Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Clinical Medicine & Laboratory Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, Jiangsu, P.R.. China Abstract Bladder cancer has high morbidity and mortality, and cigarette smoke is a major risk factor for this disease.. Exosomal circRNAs are widely involved in tumor progression, while their roles in smoke-related bladder cancer remain unclear.

    Article Title: Chemokine-Like Receptor 1 Knockdown Suppresses Oral Squamous Cell Carcinoma Progression by Reducing Oxidative Phosphorylation
    Article Snippet: Equal amounts of protein were separated through 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Millipore, Burlington, MA, USA). .. These membranes were then blocked with 5% blocking buffer in Tris-buffered saline with 0.1% Tween 20 for 1 hour at room temperature, with this followed by overnight incubation at 4 °C with primary antibodies against CMKLR1 (#ab306554; Abcam, Cambridge, UK), β-actin (# P30002 ; Abmart, Shanghai, China), E-cadherin (#610181; BD Biosciences, Shanghai, China), N-cadherin (#13116; Cell Signaling Technology, Danvers, MA, USA), vimentin (#5741; Cell Signaling Technology), Snail (#3879; Cell Signaling Technology), Slug (#9585; Cell Signaling Technology), PI3K (#AF6241; Affinity Biosciences, Cincinnati, OH, USA), PGC1 (#AF5395; Affinity Biosciences), Phospho-PI3K (#AF3241; Affinity Biosciences), and OXPHOS Cocktail (#PK30006; Proteintech, Rosemont, IL, USA). .. After the membranes were washed, they were incubated with secondary antibodies conjugated to horseradish peroxidase (#ab6721; Abcam and #31430; Invitrogen) for 1 hour at room temperature.

    Article Title: Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptake
    Article Snippet: .. Sections were incubated overnight at 4 °C with primary antibodies against CD31 (Servicebio, Cat# GB120005 , 1:200), Vimentin (CST, Cat# 5741, 1:200), and 8-hydroxyguanosine (8-OHG, Rockland, Cat# 200-301-A99, 1:200). .. Sections were incubated overnight at 4 °C with primary antibodies against CD31 (Servicebio, Cat# GB120005 , 1:200), Vimentin (CST, Cat# 5741, 1:200), and 8-hydroxyguanosine (8-OHG, Rockland, Cat# 200-301-A99, 1:200).

    Cell-Signaling:

    Article Title: Exosomal circ0000549 promotes MNNG-induced gastric cancer through miR-15b-5p/KIF1B
    Article Snippet: .. The membranes were blocked with 5% skimmed milk for 1 h at room temperature and incubated overnight at 4°C with primary antibodies against N-cadherin (Cell Signaling Technology, Inc; cat. no. 13116S; dilution: 1:1,000), E-cadherin (Cell Signaling Technology, Inc; cat. no. 3195S; dilution: 1:1,000), PCNA (Cell Signaling Technology, Inc.; cat. no. 13110S; dilution: 1:1,000), Nanog (Cell Signaling Technology, Inc.; cat. no. 4903S; dilution: 1:1,000), OCT4 (Cell Signaling Technology, Inc; cat. no. 2750; dilution: 1:1,000), Lin28 (Cell Signaling Technology, Inc.; cat. no. 8706S; dilution: 1:1,000), SOX2 (Cell Signaling Technology, Inc; cat. no. 23064S; dilution: 1:1,000), Vimentin (Cell Signaling Technology, Inc.; cat. no. 5741S; dilution: 1:1,000), HSP70 (Cell Signaling Technology, Inc.; cat. no. 4872S; dilution: 1:1,000), Alix (Cell Signaling Technology, Inc.; cat. no. 92880S; dilution: 1:1,000), TSG101 (Proteintech, Inc; cat. no. 28283-1-AP; dilution: 1:1,000), Calnexin (Cell Signaling Technology, Inc.; cat. no. 2679S; dilution: 1:1,000), KIF1B (Abcam; cat. no. ab72108; dilution:1:1,500) and GAPDH (Bioworld Technology, Inc.; cat. no. BS65483M; dilution: 1:2,000). .. After washing with TBST containing 0.1% Tween-20, membranes were incubated with appropriate horseradish peroxidase-conjugated secondary antibodies (Signalway Antibody LLC; cat. no. L3012; dilution: 1:5,000).

    Article Title: Chemokine-Like Receptor 1 Knockdown Suppresses Oral Squamous Cell Carcinoma Progression by Reducing Oxidative Phosphorylation
    Article Snippet: Equal amounts of protein were separated through 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Millipore, Burlington, MA, USA). .. These membranes were then blocked with 5% blocking buffer in Tris-buffered saline with 0.1% Tween 20 for 1 hour at room temperature, with this followed by overnight incubation at 4 °C with primary antibodies against CMKLR1 (#ab306554; Abcam, Cambridge, UK), β-actin (# P30002 ; Abmart, Shanghai, China), E-cadherin (#610181; BD Biosciences, Shanghai, China), N-cadherin (#13116; Cell Signaling Technology, Danvers, MA, USA), vimentin (#5741; Cell Signaling Technology), Snail (#3879; Cell Signaling Technology), Slug (#9585; Cell Signaling Technology), PI3K (#AF6241; Affinity Biosciences, Cincinnati, OH, USA), PGC1 (#AF5395; Affinity Biosciences), Phospho-PI3K (#AF3241; Affinity Biosciences), and OXPHOS Cocktail (#PK30006; Proteintech, Rosemont, IL, USA). .. After the membranes were washed, they were incubated with secondary antibodies conjugated to horseradish peroxidase (#ab6721; Abcam and #31430; Invitrogen) for 1 hour at room temperature.

    other:

    Article Title: Network pharmacology reveals that Yanghe Decoction inhibits osteosarcoma progression via ROS-induced mitochondrial dysfunction and enhances cisplatin sensitivity
    Article Snippet: Proliferating cell nuclear antigen (PCNA) (#F0018), B-cell lymphoma 2 (Bcl-2)-associated X protein (Bax) (#F0037), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (#F0003) antibodies were purchased from Selleck (USA).

    Blocking Assay:

    Article Title: Multi-Omics and Functional Analyses Identify let-7b-3p as a Negative Regulator of EMT in Lung Adenocarcinoma.
    Article Snippet: Lung adenocarcinoma (LUAD) is the most common subtype of non‐small cell lung cancer (NSCLC), and its malignant progression involves complex molecular mechanisms.. While microRNAs (miRNAs) play a crucial regulatory role in LUAD development, their specific mechanisms remain unclear.. This study used bioinformatics analysis to identify key miRNA‐mRNA interaction axes in LUAD, revealing that let‐7b‐3p was significantly downregulated.

    Article Title: Exosomal circ55033 promotes cigarette smoke-induced bladder cancer by modulating EMT and stem cell properties.
    Article Snippet: 1 Department of Urology, The Second Affiliated Hospital of Anhui Medical University, Hefei 230032, China 2 Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Clinical Medicine & Laboratory Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, Jiangsu, P.R.. China Abstract Bladder cancer has high morbidity and mortality, and cigarette smoke is a major risk factor for this disease.. Exosomal circRNAs are widely involved in tumor progression, while their roles in smoke-related bladder cancer remain unclear.

    Article Title: Chemokine-Like Receptor 1 Knockdown Suppresses Oral Squamous Cell Carcinoma Progression by Reducing Oxidative Phosphorylation
    Article Snippet: Equal amounts of protein were separated through 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Millipore, Burlington, MA, USA). .. These membranes were then blocked with 5% blocking buffer in Tris-buffered saline with 0.1% Tween 20 for 1 hour at room temperature, with this followed by overnight incubation at 4 °C with primary antibodies against CMKLR1 (#ab306554; Abcam, Cambridge, UK), β-actin (# P30002 ; Abmart, Shanghai, China), E-cadherin (#610181; BD Biosciences, Shanghai, China), N-cadherin (#13116; Cell Signaling Technology, Danvers, MA, USA), vimentin (#5741; Cell Signaling Technology), Snail (#3879; Cell Signaling Technology), Slug (#9585; Cell Signaling Technology), PI3K (#AF6241; Affinity Biosciences, Cincinnati, OH, USA), PGC1 (#AF5395; Affinity Biosciences), Phospho-PI3K (#AF3241; Affinity Biosciences), and OXPHOS Cocktail (#PK30006; Proteintech, Rosemont, IL, USA). .. After the membranes were washed, they were incubated with secondary antibodies conjugated to horseradish peroxidase (#ab6721; Abcam and #31430; Invitrogen) for 1 hour at room temperature.

    Saline:

    Article Title: Chemokine-Like Receptor 1 Knockdown Suppresses Oral Squamous Cell Carcinoma Progression by Reducing Oxidative Phosphorylation
    Article Snippet: Equal amounts of protein were separated through 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Millipore, Burlington, MA, USA). .. These membranes were then blocked with 5% blocking buffer in Tris-buffered saline with 0.1% Tween 20 for 1 hour at room temperature, with this followed by overnight incubation at 4 °C with primary antibodies against CMKLR1 (#ab306554; Abcam, Cambridge, UK), β-actin (# P30002 ; Abmart, Shanghai, China), E-cadherin (#610181; BD Biosciences, Shanghai, China), N-cadherin (#13116; Cell Signaling Technology, Danvers, MA, USA), vimentin (#5741; Cell Signaling Technology), Snail (#3879; Cell Signaling Technology), Slug (#9585; Cell Signaling Technology), PI3K (#AF6241; Affinity Biosciences, Cincinnati, OH, USA), PGC1 (#AF5395; Affinity Biosciences), Phospho-PI3K (#AF3241; Affinity Biosciences), and OXPHOS Cocktail (#PK30006; Proteintech, Rosemont, IL, USA). .. After the membranes were washed, they were incubated with secondary antibodies conjugated to horseradish peroxidase (#ab6721; Abcam and #31430; Invitrogen) for 1 hour at room temperature.



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    Image Search Results


    FLLL32 inhibits CRC cell proliferation and migration. a Representative EdU staining images of HCT116 and HT29 cells treated with DMSO or FLLL32. Scale bars = 100 μm. b Quantification of EdU-positive cells in HCT116 and HT29 cells. c Wound-healing images of HCT116 treated with FLLL32 or DMSO. Scale bars = 20 μm. d Quantification of wound-healing distance ratio in HCT116 cells. e Western blot analysis of vimentin, E-cadherin and N-cadherin in HCT116 and HT29 cells treated with the indicated concentrations of FLLL32. β-Tubulin was used as the loading control. f Representative flow cytometry plots showing cell-cycle distribution in HCT116 and HT29 treated with FLLL32 or DMSO for 48 h. g Quantification of cell-cycle distribution in HCT116 and HT29 cells. Data are presented as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001

    Journal: Molecular Biomedicine

    Article Title: Curcumin analogue FLLL32 induces ferroptosis by targeting the STAT3-GPX4/SLC40A1 axis in colorectal cancer

    doi: 10.1186/s43556-026-00575-5

    Figure Lengend Snippet: FLLL32 inhibits CRC cell proliferation and migration. a Representative EdU staining images of HCT116 and HT29 cells treated with DMSO or FLLL32. Scale bars = 100 μm. b Quantification of EdU-positive cells in HCT116 and HT29 cells. c Wound-healing images of HCT116 treated with FLLL32 or DMSO. Scale bars = 20 μm. d Quantification of wound-healing distance ratio in HCT116 cells. e Western blot analysis of vimentin, E-cadherin and N-cadherin in HCT116 and HT29 cells treated with the indicated concentrations of FLLL32. β-Tubulin was used as the loading control. f Representative flow cytometry plots showing cell-cycle distribution in HCT116 and HT29 treated with FLLL32 or DMSO for 48 h. g Quantification of cell-cycle distribution in HCT116 and HT29 cells. Data are presented as the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001

    Article Snippet: Primary antibodies used for immunoblot analysis were as follows: β-Tubulin (ZSGB-BIO, TA347064), STAT3 (HUABIO, ET1603-40), Phospho-STAT3 (Tyr705) (CST, 9145S), GPX4 (HUABIO, ET1706-45), SLC40A1 (CST, 80672T), FTH1 (Abclonal, A1144), FTL (Abclonal, A1768), Lamin B1 (Abclonal, A11495), E-cadherin (Proteintech, 20874-1-AP), N-cadherin (Proteintech, 22018-1-AP), Vimentin (Proteintech, 10366-1-AP), and HA-tag (Proteintech, 66006-2-Ig).

    Techniques: Migration, Staining, Western Blot, Analysis, Control, Flow Cytometry

    Journal: iScience

    Article Title: NAT10 exacerbates aortic valve calcification via PCSK9 mRNA ac4C acetylation

    doi: 10.1016/j.isci.2026.117812

    Figure Lengend Snippet:

    Article Snippet: anti-Vimentin , Proteintech , Cat#60330-1-Ig; RRID: AB_2881439.

    Techniques: